2316 45H. SAKAMOTO and Y. HASHIMOTO cargo. On the other hand, the results for the wasabi-sheet container are summarized as follows: Under the condition with the minimum number of wasabi sheets per cardboard box, only one out of 128 RIFA workers survived (survival rate 0.01), while all RIFA workers died under the other conditions (Fig. 5b). No RIFA were found alive inside the cardboard boxes. These results indicated that wasabi sheets demonstrated a strong repellent activity against RIFA workers during the seven-day transport period typically required for shipping between Asian countries. 112 3sheet/box6sheet/box9sheet/box12sheet/box15sheet/box20ftseacontainerFirst, the results for the control container are summarized as follows: 80% of the RIFA workers survived for seven days with only water supplied (N=1,000) (Fig. 5a). Over 10% of the cardboard boxes were found to have been invaded by RIFA workers (N=60). The majority of the surviving RIFA workers were discovered in the tray at the bottom of the tower, where the water supply was available. These results confirmed the previously understood risk of RIFA invasion in sea containers. RIFA can survive several days of transport as long as water is available, allowing them to invade the 1-A2-ADoor1-B2-B3-A4-A3-B4-B5.9 m32.5 cm25.5cm(x6=153.0cm)1.2 m5-A2.4 m5-B46.5 cm*AspecifiednumberofWASABI-sheetswereplacedineachcardboardbox.S=0.78(N=59)S=0.00(N=100)Sideview2.4 mDoorTopviewTubeGasbarriorfilmFig. 4 Experimental design of the RIFA repellent transportation experiment. Fig. 5 One set of survival rates of RIFA after seven days in the container experiment. Blue numbers the number of surviving indicate individuals, while red numbers indicate the number of deceased individuals. The survival rate is denoted by the abbreviation ‘S’; a) control. b) with WASABI-Sheet. a)S=0.84(N=57)S=0.90(N=53)b)S=0.02(N=66)S=0.00(N=100)S=0.81(N=97)S=0.84(N=80)S=0.00(N=100)S=0.00(N=100)
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